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KMID : 0603520030080010030
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Journal of Korean Association of Cancer Prevention
2003 Volume.8 No. 1 p.30 ~ p.35
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Biological Function of Selenoprotein W: a Glutathione-Dependent Antioxidant
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Kim Ick-Young
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Abstract
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The function of selenoprotein W (Se-W) was investigated by cloning the corresponding cDNA from mouse brain and expressing it in CHO ceIls and Hl299 human lung cancer cells. Overexpression of Se-W markedly reduced the sensitivity of both cell lines to H2O2 cytotoxicity. The intracellular peroxide concentration of the transfected cells was lower than that of the parental cells in the absence or presence of extracellular H2O2. The resistance to oxidative stressconferred by Se-W was dependent on glutathione. Expression of Se-W mutants in which selenocysteine-13 or cysteine-37 was replaced by serine did not confer resistance to H2O2, implicating these residues in the antioxidant activity of Se-W in vivo.
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KEYWORD
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Selenium, Selenoprotein W, GSH, Antioxidant
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